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AFB is a three-letter acronym that may refer to:
- AzFinansBank - AFB Bank
- Acid-fast bacilli
- Ana Fa5oor Beek
- Air Force Base
- Automatische Fahr- und Bremssteuerung, speed control for trains
- American Farm Bureau
- American foulbrood, a honeybee disease
- American Foundation for the Blind
- SIL code for spoken Gulf Arabic
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