出典: フリー百科事典『ウィキペディア(Wikipedia)』

出典: フリー百科事典『ウィキペディア(Wikipedia)』






初登場はハエ (1993)である[1]。のちにゼブラフィッシュでも開発された[2][3]


GAL4/UASシステムに用いられるUASは主に酵母のGal1・Gal10を制御するUAS (UASG)である。UASGは複数のGAL4結合領域からなっている。GAL4/UASシステムはそのうち一部を利用している。

type sequence species finder/developer usecase
CGGATTAGAAGCCGCCG yeast Giniger, et al., 1985.[4]
CGGGTGACAGCCCTCCG yeast Giniger, et al., 1985.[4]
AGGAAGACTCTCCTCCG yeast Giniger, et al., 1985.[4]
CGCGCCGCACTGCTCCG yeast Giniger, et al., 1985.[4]
near-consensus synthetic 17 bp CGGAAGACTCTCCTCCG synthetic[5] Giniger, et al., 1985.[4]
17M CGGAGTACTGTCCTCCG synthetic[6] Webster, et al.. 1988.[7] pT2RUASGCaMP7a (5x)[8], pUAST (5x, fly gene expression), pUASTattB (5x, fly gene expression), pMF3 (10x, GD RNAi library in Vienna Drosophila Resource Center)

その他のUASG: [9]


UASのサイレンシングが問題になる[10]. 至適UASリピート数には議論がある[11]


浅川 和秀・川上 浩一. 「Tol2トランスポゾンを用いたゼブラフィッシュGAL4エンハンサートラップ法の確立」[12]


  1. ^ To create GAL4-responsive target genes, we designed a vector into which genes can be subcloned behind a tandem array of five optimized GAL4 binding sites (hereafter referred to as the UAS, for Upstream Activation Sequence)
  2. ^ The coding region is under the control of five copies of an optimized UAS from yeast and the minimal E1b promoter of adenovirus (Argenton et al., 1996).   "Use of the Gal4-UAS technique for targeted gene expression in the zebrafish"   Nico ScheerJosé A. Campos-Ortega. 1999.
  3. ^ "An activation domain of the helix-loop-helix transcription factor E2A shows cell type preference in vivo in microinjected zebra fish embryos."   F Argenton, Y Arava, A Aronheim, and M D Walker. 1996.   UASLineは貰ったよ、くらいしか記述なし。
  4. ^ a b c d e
  5. ^ GAL4認識配列の発見を証明するため、人工的な認識配列を作製 > To test the assertion that we have identified the GAL4 protein recognition sequence, we synthesized an “improved” site containing the innermost 15 bp of the natural site 3, but with the outer two base pairs altered to agree with our consensus sequence (Figure 7).
  6. ^ The sequence of this binding site is 5'-CGGAGTACTGTCCTCCG-3’ and contains a single base change (G->T) from the perfect palindromic consensus to create a ScaⅠ site. >
  7. ^
  8. ^
  9. ^
  10. ^ Each UAS is 17 base pairs long, roughly palindromic, and in the form of CGG-N11-CCG. The CpG dinuleotides are essential for Gal4 binding (Marmorstein et al., 1992) and serve as a target for methylation (Goll et al., 2009).
  11. ^
  12. ^